Journal: Cell

Article Title: RalB GTPase-mediated activation of the IkappaB family kinase TBK1 couples innate immune signaling to tumor cell survival.

doi: 10.1016/j.cell.2006.08.034

Figure Lengend Snippet: Figure 6. RalB and Sec5 Are Required for Host Defense Pathway Activation (A) RalGTPases are activated in response to TLR3 signaling. HBECs were treated with poly(I:C) (100 mg/ml) for the indicated time points (0, 5, 10, 15 min). GST-Sec5rbd was used to immobilize endogenous GTP-bound RalA and RalB. Phospho-IRF-3 (Ser396) from whole-cell lysates (WCL) is shown to indicate responsiveness to poly (I:C). (B) RasGTPases are inactivated in response to TLR3 signaling. HBECs were treated as in (A). GST-Raf1rbd was used to immobilize endogenous GTP-bound Ras. (C) RalB is activated in response to Sendai virus infection. HBECs were exposed to Sendai virus (100 HA/ml) for the indicated times and assessed for RalB activation as in (A). (D) RalB and Sec5 are required for IRF-3 but not p65 responsiveness to TLR3 activation. HBECs were cotransfected with a plasmid expressing GFP together with the indicated siRNAs. This method delivers RNA and DNA into a small subset of the population, which can be monitored by GFP expression and allows observation of potentially defective responses within a background of appropriately responding cells. Seventy-two hours

Article Snippet: The following antibodies were used: mouse monoclonal anti-RalA and rabbit polyclonal anti-RalB (BD, Transduction Laboratories); rabbit polyclonal ERK1/2 (Santa Cruz Biotechnology); rabbit polyclonal anti-Sec5, mouse monoclonal anti-Sec6; rabbit polyclonal anti-Sec10, rabbit polyclonal anti-Exo84 (Moskalenko et al., 2002, 2003); rabbit polyclonal anti-IRF-3 (Santa Cruz Biotechnology); rabbit polyclonal anti-phospho-IRF-3 (Ser396) (Upstate); and mouse monoclonal anti-TBK1/NAK (Imgenex), mouse monoclonal anti-Ras (BD), and rabbit polyclonal anti-GFP (Santa Cruz Biotechnology).

Techniques: Activation Assay, Virus, Infection, Plasmid Preparation, Expressing

Journal: iScience

Article Title: A p53-TLR3 axis ameliorates pulmonary hypertension by inducing BMPR2 via IRF3

doi: 10.1016/j.isci.2023.105935

Figure Lengend Snippet:

Article Snippet: p-IRF3 , Abnova , PAB25053.

Techniques: Clone Assay, Isolation, Recombinant, Plasmid Preparation, Membrane, Microscopy, Electron Microscopy, Amplification, Reverse Transcription, SYBR Green Assay, Software

Journal: Cell

Article Title: FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells

doi: 10.1016/j.cell.2020.11.042

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-human phosphorylated-IRF3 S386 , GeneTex , Cat# GTX130422.

Techniques: Control, Marker, Microarray, Recombinant, Plasmid Preparation, Imaging, Staining, Extraction, Immunoprecipitation, Cell Isolation, Knockdown, Software